胃癌组织环状RNA-UBAP2和UBAP2蛋白的表达及临床意义

张友满; 曾潘; 阮稳稳; 徐初锋

doi:10.12019/j.issn.1671-5144.2020.04.008

胃癌组织环状RNA-UBAP2和UBAP2蛋白的表达及临床意义

1.
安康市中心医院普外科, 陕西安康 725000
2.
安康市中医医院普外科, 陕西安康 725000
3.
安康市人民医院肿瘤科, 陕西安康 725000

详细信息

作者简介:
张友满（1989-）,男,陕西安康人,主治医师,医学硕士,研究方向为胃肠肿瘤综合治疗。

通讯作者:
徐初锋,Tel: 0915-3284060, E-mail: gaoxing194@163.com

中图分类号: R735.2
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出版历程
- 收稿日期: 2020-08-10
- 发布日期: 2020-08-27

Expression of Circular RNA-UBAP2 and UBAP2 Protein in Gastric Cancer and Its Clinical Significance

1.
Department of General Surgery, Ankang Central Hospital, Shanxi Ankang 725000, China
2.
Department of General Surgery, Ankang Traditional Chinese Medicine Hospital, Shanxi Ankang 725000, China
3.
Department of Oncology, Ankang People's Hospital, Shanxi Ankang 725000, China

摘要

摘要: 目的探讨胃癌患者胃癌组织中和外周血中环状RNA-UBAP2（ubiquitin associated protein 2）和UBAP2蛋白的表达及临床意义。方法选取2019年2月至2020年2月于我院行手术治疗的100例胃癌患者纳入研究,收集术后胃癌患者和正常人的外周血、胃癌组织及癌旁组织作为样本,采用qRT-PCR法检测样本组织中环状RNA-UBAP2表达,并分析其与患者临床特征的关系;采用Western blot技术检测胃癌组织及癌旁组织中UBAP2蛋白的表达。结果胃癌组织中环状RNA-UBAP2的相对表达量为（8.73±1.04）,明显高于癌旁组织（5.12±0.85）,二者比较差异显著（P<0.001）;胃癌患者的外周血样本中环状RNA-UBAP2的相对表达量为（7.55±0.65）,明显高于正常人外周血（5.08±0.23）,二者比较差异显著（P<0.001）;胃癌组织中环状RNA-UBAP2的表达与淋巴结转移、临床分期、浸润深度、远端转移及脉管癌栓有关（P<0.05）;环状RNA-UBAP2诊断胃癌的曲线下面积为0.813（95%CI 0.729~0.934）,约登指数为0.406,诊断特异度为82.15%,敏感度为91.10%;胃癌组织UBAP2蛋白的表达明显高于癌旁组织。结论胃癌组织中及胃癌患者外周血环状RNA-UBAP2呈高表达,胃癌组织UBAP2蛋白也呈高表达,其可能成为临床胃癌诊断的新指标。
- 胃癌 /
- 环状RNA-UBAP2 /
- 表达 /
- 临床意义
Abstract: Objective To explore the expression and clinical significance of circular RNA-UBAP2（ubiquitin associated protein 2） and UBAP2 protein in gastric cancer tissues and peripheral blood of patients with gastric cancer. Methods Selected 100 gastric cancer patients who underwent surgical treatment in our hospital from February 2019 to February 2020 to be included in the study. Collect peripheral blood, gastric cancer tissues and adjacent tissues of gastric cancer patients and normal people after surgery as samples, and use qRT-PCR to detect samples' cyclic RNA-UBAP2 expression in tissues, and analyzed its relationship with the clinical characteristics of patients. Western blot technology was used to detect the expression of UBAP2 protein in gastric cancer tissues and adjacent tissues. Results The relative expression of circular RNA-UBAP2 in gastric cancer tissues was （8.73±1.04）, significantly higher than that of adjacent tissues （5.12±0.85）, （P<0.001）; The relative expression of cyclic RNA-UBAP2 was （7.55±0.65）, which was significantly higher than that of normal human peripheral blood （5.08±0.23）, （P<0.001）; the expression of circular RNA-UBAP2 in gastric cancer lymph node metastasis, clinical stage, depth of invasion, distal metastasis and vascular tumor thrombus were related （P<0.05）; the area under the curve for the diagnosis of gastric cancer by circular RNA-UBAP2 was 0.813 （95%CI 0.729~0.934）, Youden index was 0.406, the diagnostic specificity was 82.15%, and the sensitivity was 91.10%; the expression of UBAP2 protein in gastric cancer tissue was significantly higher than that in adjacent tissues. Conclusions Circular RNA-UBAP2 was highly expressed in gastric cancer tissues and in the peripheral blood of patients with gastric cancer, and UBAP2 protein was also highly expressed in gastric cancer tissues, which may become a new index for clinical diagnosis of gastric cancer.
- gastric cancer /
- circular RNA-UBAP2 /
- expression /
- clinical significance

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参考文献(16)

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施引文献(2)

期刊类型引用(2)

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2.	陈婧，张荣，王浩斌，邱昌伟. 血清circNRIP1表达在胃癌诊疗中的临床意义. 胃肠病学. 2021(01): 39-42 . 百度学术