Objective To explore the factors influencing the detection rate of human papillomavirus (HPV) cervical cancer and the application of in vitro qualitative HPV-DNA test in HPV16/18 shunt screening.

Methods The results of 5 217 patients with in vitro qualitative HPV-DNA nucleic acid testing were analyzed by stratified cluster random sampling cross-sectional investigation. The clinical data of patients were recorded and the risk factors affecting the positive detection rate of HPV cervical cancer were analyzed. The nomogram model was constructed based on the risk factors, and the application effect of the model was evaluated by the receiver operating characteristic (ROC) curve and calibration curve.

Results A total of 5 217 patients were screened by vaginal in vitro qualitative HPV cervical cancer screening mode and pathological diagnosis by colposcopy. The results showed that there were 2 187 true positive cases, 2 248 true negative cases, 495 false positive cases, and 287 false negative cases. The accuracy, sensitivity, specificity, positive predictive value, and negative predictive value of HPV were 85.01%, 88.40%, 81.95%, 81.54%, and 88.68%, respectively. Age > 45 years, diabetes mellitus, hypertension, hyperlipidemia, endometritis, mammary hyperplasia, vaginitis, ovarian cysts, and mastitis influenced the positive detection rate of HPV-DNA cervical cancer. The ROC curve and calibration curve showed that the model had a good application effect.

Conclusions In vitro vaginal HPV DNA cervical cancer screening has high accuracy and can be used in shunt screening for HPV16/18 types.