LncRNA DANCR调控食管癌细胞增殖、转移及其miR-216a表达的研究

周爱国; 郑梦利; 张书新; 周鹏宇

doi:10.12019/j.issn.1671-5144.202305043

LncRNA DANCR调控食管癌细胞增殖、转移及其miR-216a表达的研究

Research on lncRNA DANCR Regulation of Proliferation, Metastasis, and miR-216a Expression in Esophageal Cancer Cells

摘要

摘要:
目的探讨长链非编码RNA分化拮抗非蛋白编码（long non-coding RNA differentiation antagonizing nonprotein coding RNA，lncRNA DANCR）调控食管癌细胞增殖、转移及miR-216a表达的影响。
方法本研究通过慢病毒转染技术构建了过表达及敲低lncRNA DANCR的食管癌细胞株，随后通过CCK-8实验、划痕实验和Transwell实验来验证lncRNA DANCR对食管癌细胞增殖、迁移和侵袭的影响。荧光素酶报告基因实验检测食管癌细胞中lncRNA DANCR对miR-216a表达的抑制作用。
结果 LncRNA DANCR过表达促进了食管癌细胞的增殖、迁移和侵袭作用，而敲低则削弱了其增殖和转移作用。逆转录聚合酶链反应（reverse transcription polymerase chain reaction，RT-PCR）实验结果显示lncRNA DANCR抑制了miR-216a的表达，荧光素酶报告基因实验的结果也证实了这一结果。
结论 LncRNA DANCR能够正向调控食管癌细胞增殖、迁移和侵袭，但与miR-216a表达呈负相关性。

Abstract:
Objective To explore the effect of long non-coding RNA differentiation antagonizing nonprotein coding RNA (lncRNA DANCR) on promoting proliferation and metastasis of esophageal cancer cells by inhibiting the expression of miR-216a.
Method This study constructed esophageal cancer cell lines overexpressing and knocking down lncRNA DANCR using lentiviral transfection technology. Subsequently, the effects of lncRNA DANCR on the proliferation, migration, and invasion of esophageal cancer cells were verified through CCK-8 assay, scratch assay, and Transwell assay. The luciferase reporter gene assay was used to detect the inhibitory effect of lncRNA DANCR on miR-216a expression in esophageal cancer cells.
Results Overexpression of lncRNA DANCR promoted the proliferation, migration, and invasion of esophageal cancer cells, while knockdown weakened their proliferation and metastasis. The results of the reverse transcription polymerase chain reaction (RT-PCR) experiment showed that lncRNA DANCR inhibited the expression of miR-216a, and the results of the luciferase reporter gene experiment also confirmed this result.
Conclusion LncRNA DANCR can positively regulate the proliferation, migration, and invasion of esophageal cancer cells, but it is negatively correlated with miR-216a expression.

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