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    MiR-181a-5p调控乳头状甲状腺癌细胞增殖和侵袭的作用及分子机制研究

    Effect and Molecular Mechanism of MiR-181a-5p on the Proliferation and Invasion of Papillary Thyroid Cancer Cells

      摘要
    • 摘要:
      目的 探讨miR-181a-5p对乳头状甲状腺癌（papillary thyroid cancer，PTC）细胞的作用及调控机制。
      方法 利用生物信息学工具分析miR-181a-5p和促甲状腺激素受体（thyrotropin receptor，TSHR）在PTC组织中的表达以及两者的靶向关系，并使用双荧光素酶报告基因实验验证两者的靶向关系。TPC-1细胞转染miR-181a-5p抑制物和shTSHR后，使用定量逆转录聚合酶链反应或免疫印迹实验检测TPC-1细胞中miR-181a-5p、TSHR、E-cadherin、N-cadherin和Vimentin的表达水平。通过MTT和Transwell实验检测miR-181a-5p和TSHR对TPC-1细胞活性、侵袭的作用。
      结果 在PTC组织和细胞中miR-181a-5p表达上调（P<0.001），在甲状腺癌组织中TSHR低表达，且miR-181a-5p可直接靶向TSHR。下调miR-181a-5p抑制了TPC-1细胞活性、侵袭以及N-cadherin和Vimentin的表达（P<0.01），但促进了E-cadherin的表达（P<0.001）。TSHR敲减产生了相反的结果（P<0.01），并部分逆转了miR-181a-5p下调对TPC-1细胞的影响（P<0.01）。反之，miR-181a-5p下调可以逆转TSHR敲减产生的影响（P<0.05）。
      结论 下调miR-181a-5p可通过促进TSHR的表达来抑制PTC细胞的恶性进展。

       

      Abstract:
      Objective To investigate the effect and regulation mechanism of miR-181a-5p on papillary thyroid carcinoma (PTC) cells.
      Methods The expressions of miR-181a-5p and thyrotropin receptor (TSHR) in PTC tissues, and the targeting relationship between these two genes were analyzed using bioinformatics tools, and their relationship was verified by dual-luciferase reporter assay. After TPC-1 cells transfected with miR-181a-5p inhibitor and shTSHR, the expression levels of miR-181a-5p, TSHR, E-cadherin, N-cadherin and Vimentin were detected by quantitative reverse transcription polymerase chain reaction or western blot assay. The effects of miR-181a-5p and TSHR on the viability and invasion of TPC-1 cells were detected by MTT and Transwell assay.
      Results The expression of miR-181a-5p was up-regulated in PTC tissues and cells (P<0.001), and the expression of TSHR was low in thyroid carcinoma tissues, and miR-181a-5p could directly target TSHR. Down-regulation of miR-181a-5p inhibited viability and invasion, and the expression of N-cadherin and Vimentin (P<0.01), but promoted the expression of E-cadherin in TPC-1 cells (P<0.001). TSHR knockdown produced the opposite result (P<0.01), and partially reversed the effect of miR-181a-5p downregulation on TPC-1 cells (P<0.01). Conversely, down-regulation of miR-181a-5p could reverse the effect of TSHR knockdown (P<0.05).
      Conclusions Downregulation of miR-181a-5p inhibited the malignant progression of PTC cells by promoting the expression of TSHR.

       

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