Relationships between Polymorphisms of Laminin 5 and Expression Level in Lung Cancer Cell Lines

Objective To explore the relationships between the polymorphisms in the 5′-UTR region of laminin 5 （LN5） and the expression level in lung cancer cell lines. Methods The polymorphisms of LN5 and the mRNA expression levels in 12 lung cancer cell lines were detected, using sequencing and real-time PCR methods, respectively, the relationships between them were analyzed. Results Cell lines with GA genotype of -183G/A polymorphisms have a higher mRNA expression level of LN5 than the cell lines with GG genotype （t= 7.136,P<0.001）. No relationships of -6C/A and -89A/G polymorphisms and the mRNA expression levels were found in this study. Conclusions This study suggestes that -183G/A polymorphisms of LN5 gene maybe regulate the transcriptional level, and these results provide the basic evidence for the further mechanism study.