Abstract: Current pathology staining training often suffers from insufficient biosecurity awareness and non-standardized operations among students. To address these shortcomings, this paper conducts a systematic analysis of the existing teaching system, identifying critical gaps in four key areas: operational protocols, personnel competency, pedagogical approach, and management oversight. Grounded in the principles of biosecurity risk control, we propose a comprehensive reform model that reconstructs a mutually reinforcing, closed-loop teaching ecosystem through four integrated dimensions: curriculum content, teaching methodologies, evaluation mechanisms, and administrative support. This initiative deeply integrates biosecurity principles into the entire practical training process, consolidates educational resources, and optimizes practical evaluation systems. The ultimate aim is to systematically enhance the biosecurity literacy and standardized operational skills of medical students, thereby laying a solid foundation for the safe and efficient conduct of scientific research. This model is intended to provide a referable new paradigm for improving safety management in experimental teaching within medical colleges.