Effect of Combining Ischemic Pretreatment and Ischemic Postconditioning on Pressure Ulcer Induced by Ischemia-Reperfusion Injury in Rats
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摘要: 目的 通过建立大鼠压疮缺血再灌注性损伤缺血处理模型,比较缺血联合处理方法对大鼠皮肤及皮下组织的保护作用,为临床判断压疮损伤的程度及压疮干预效果提供理论依据。 方法 将60只SD大鼠随机分为对照组、缺血再灌注组、缺血联合处理组,每组20只,建立大鼠压疮缺血再灌注性损伤处理模型,通过肉眼观察受压程度,检测血清氧自由基的水平,以判断压疮缺血再灌注损伤程度。 结果 缺血再灌注组、缺血联合处理组大鼠出现Ⅰ期压疮的发生率为100%;缺血联合处理组血清一氧化氮[（40.2±1.3） μmol/L]、丙二醛[（5.3±0.4） nmol/mL]含量均高于对照组（P<0.005）,明显低于缺血再灌注组,超氧化物歧化酶活性[（122.0±7.3）U/mL]低于对照组,明显高于缺血再灌注组（P<0.001）。 结论 缺血联合处理对压疮缺血再灌性损伤具有保护作用,该联合处理方法在降低丙二醛和一氧化氮水平、增加超氧化物歧化酶活性方面无明显的协同作用和叠加效果。Abstract: Objectives To build pressure ulcer model induced by ischemia-reperfusion injury in rats, compare the protective effect of pretreatment, posttreatment and combined treatment of reperfusion injury on rat skin and subcutaneous tissue, and provide a theoretical basis for the assessment of injurious degree and intervention effect of pressure ulcer in clinic. Methods 60 Sprague-Dawlye rats were equally randomized divided into three groups: control group （group S）, ischemia-reperfusion group （group IR）, and ischemic pretreatment and ischemic postconditioning group （group combined）. The extent of decompression was observed and the oxygen free radical was detected to assess the injurious degree of pressure ulcer in the model. Results The incidence of pressure ulcer at stageⅠin group Combined and group IR were 100%. The serum concentration of nitric oxide （NO）（40.2±1.3） μmol/L and and group IR malondialdehyde（MDA）（5.3±0.4） nmol/mL in group combined were higher than that in group S（P<0.005）. The activity of superoxide dismutasein（SOD）（122.0±7.3）U/mL in group combined were higher than that in group IR but lower than that in group S（P<0.001）. Conclusions Combined ischemic treatment has a protective effect on pressure ulcer induced by ischemia reperfusion injury. But it has no obvious synergy effect neither in decreasing the levels of MDA, NO nor in increasing the activity of SOD.
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