Abstract: Objectives To build pressure ulcer model induced by ischemia-reperfusion injury in rats, compare the protective effect of pretreatment, posttreatment and combined treatment of reperfusion injury on rat skin and subcutaneous tissue, and provide a theoretical basis for the assessment of injurious degree and intervention effect of pressure ulcer in clinic. Methods 60 Sprague-Dawlye rats were equally randomized divided into three groups: control group （group S）, ischemia-reperfusion group （group IR）, and ischemic pretreatment and ischemic postconditioning group （group combined）. The extent of decompression was observed and the oxygen free radical was detected to assess the injurious degree of pressure ulcer in the model. Results The incidence of pressure ulcer at stageⅠin group Combined and group IR were 100%. The serum concentration of nitric oxide （NO）（40.2±1.3） μmol/L and and group IR malondialdehyde（MDA）（5.3±0.4） nmol/mL in group combined were higher than that in group S（P<0.005）. The activity of superoxide dismutasein（SOD）（122.0±7.3）U/mL in group combined were higher than that in group IR but lower than that in group S（P<0.001）. Conclusions Combined ischemic treatment has a protective effect on pressure ulcer induced by ischemia reperfusion injury. But it has no obvious synergy effect neither in decreasing the levels of MDA, NO nor in increasing the activity of SOD.