Abstract: Objective To explore the diagnostic value of endobronchial ultrasound-guide sheath under virtual bronchoscopic navigation （EBUS-GS-VBN） for peripheral lung cancer. Methods 134 cases of patients whose computed tomography （CT） report highly suspected diagnosis of peripheral lung cancer from respiratory inpatient department of Hebei Petro China Central Hospital during the period of July 2018 to July 2020 were included, all patients were examined by histopathology, the diagnostic sensitivity, specificity, positive predictive value （PPV）, negative predictive value （NPV） and accuracy of lesion diameter （≥3 cm and <3 cm）, pleural distance （≥3 cm and <3 cm）, lesion properties （solid and non-solid） by EBUS-GS-VBN were analyzed, the diagnostic coincidence rate of lesion sites （upper lobe, middle lobe and lower lobe） by EBUS-GS-VBN were analyzed. Results The sensitivity, specificity, PPV, NPV and accuracy of EBUS-GS-VBN for peripheral lung cancer were respectively 98.04%, 93.75%, 98.04%, 93.75% and 97.01%. Further subgroup analysis found the diagnostic sensitivity, specificity, positive predictive value, negative predictive value and accuracy of lesion size, pleural distance, lesion properties by EBUS-GS-VBN and the diagnostic coincidence rate of the lesion site by EBUS-GS-VBN were all >90%. Conclusions The value of EBUS-GS-VBN was satisfying in the diagnosis of peripheral lung cancer, and it can accurately determine lesion size, pleural distance, lesion properties and lesion sites, which was worthy of clinical popularization.