胶质瘤中RNA结合蛋白hnRNPH1的表达及其预后意义

    Expression and Prognostic Significance of RNA-Binding Protein hnRNPH1 in Glioma

    • 摘要: 目的 探讨RNA结合蛋白异质性胞核核糖核蛋白H1(heterogeneous nuclear ribonucleoprotein H1,hnRNPH1)在胶质瘤中的表达情况及其临床意义。 方法 选取2000年1月至2012年12月于广东省人民医院收治进行手术的胶质瘤患者311例。取胶质瘤组织作为研究样本,取癌旁正常脑组织作为对照样本。利用大数据生物信息学数据库分析hnRNPH1的泛癌种表达量,采用免疫组织化学(immunohistochemictry,IHC)方法检测组织芯片中hnRNPH1在胶质瘤组织及癌旁正常组织中的表达量,卡方检验法分析胶质瘤组织中hnRNPH1的表达水平与患者临床病理参数之间的关系。单因素和多因素回归分析hnRNPH1表达水平和临床病理参数对胶质瘤患者生存的预测作用。Kaplan-Meier曲线法分析hnRNPH1表达水平与肿瘤患者生存期的关系。 结果 分析癌症和肿瘤基因图谱(Cancer Genome Atlas,TCGA)数据库显示hnRNPH1在泛癌种组织中普遍高表达,与正常组织相比,hnRNPH1在胶质母细胞瘤组织中具有更高的表达水平,且随着患者年龄增加,hnRNPH1的表达水平逐渐增加。采用IHC检测胶质瘤组织芯片hnRNPH1的表达,结果显示hnRNPH1表达水平与胶质瘤级别正相关。生存分析结果显示,高表达hnRNPH1的患者总生存期短。hnRNPH1的表达与患者年龄、性别、肿瘤定位无关(P>0.05),与肿瘤类型和肿瘤级别相关(P<0.05);hnRNPH1的表达水平、患者年龄及肿瘤级别是预后的独立预测因素(P<0.05)。 结论 胶质瘤组织中hnRNPH1的表达水平明显上调,与肿瘤患者的不良预后密切相关,可能参与胶质瘤的发生发展过程。

       

      Abstract: Objective To investigate the expression of RNA-binding protein heterogeneous nuclear ribonucleoprotein H (hnRNPH1) in glioma and its clinical significance. Methods From January 2000 to December 2012, 311 patients with glioma underwent surgery in Guangdong Provincial People's Hospital were selected. The glioma tissue was taken as the study sample, while the normal brain tissue adjacent to the cancer was taken as the control sample. hnRNPH1 protein extracted from glioma tissues and normal tissues adjacent to cancer was assessed by immunohistochemistry (IHC) in tissue microarray. hnRNPH1 protein expression in pan-canceris analyzed by big bioinformatics database. Chi-square test was used to analyze the relationship between hnRNPH1 expression level in glioma tissues and clinicopathological parameters. Univariate and multivariate regression analysis were used to analyze hnRNPH1 expression level and clinicopathological parameters for predicting survival of patients with glioma. Kaplan-Meier curve was used to analyze the relationship between hnRNPH1 expression level and survival of tumor patients. Results The Cancer Genome Atlas (TCGA) database showed that hnRNPH1 was generally highly expressed in the tumor tissues. Compared with adjacent normal tissues, hnRNPH1 had a higher expression level in glioblastoma tissues, and the expression level of hnRNPH1 gradually increased with the age of patients. Results showed that the expression level of hnRNPH1 was positively correlated with the grade of glioma. Survival analysis showed that patients with high hnRNPH1 expression had a short overall survival. hnRNPH1 expression was not correlated to patient age, sex and tumor location (P>0.05), while significantly correlated to tumor type and grade (P<0.05). hnRNPH1 expression level, patient age and tumor grade were independent predictors of prognosis (P>0.05). Conclusions The expression level of hnRNPH1 in glioma tissue was significantly up-regulated, which was closely related to the poor prognosis of tumor patients and may be involved in the occurrence and development of glioma.

       

    /

    返回文章
    返回