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连接酶链反应检测沙眼衣原体的准确性Meta分析

葛龙, 陈婵, 梁莉, 安妮, 王小琴, 田金徽

葛龙, 陈婵, 梁莉, 安妮, 王小琴, 田金徽. 连接酶链反应检测沙眼衣原体的准确性Meta分析[J]. 循证医学, 2012, 12(4): 217-223. DOI: 10.3969/j.issn.1671-5144.2012.04.012
引用本文: 葛龙, 陈婵, 梁莉, 安妮, 王小琴, 田金徽. 连接酶链反应检测沙眼衣原体的准确性Meta分析[J]. 循证医学, 2012, 12(4): 217-223. DOI: 10.3969/j.issn.1671-5144.2012.04.012
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GE Long, CHEN Chan, LIANG Li, AN Ni, WANG Xiao-qin, TIAN Jin-hui. The Accuracy for Chlamydia Trachomatis Test by Ligase Chain Reaction: A Meta-Analysis[J]. Journal of Evidence-Based Medicine, 2012, 12(4): 217-223. DOI: 10.3969/j.issn.1671-5144.2012.04.012
Citation: GE Long, CHEN Chan, LIANG Li, AN Ni, WANG Xiao-qin, TIAN Jin-hui. The Accuracy for Chlamydia Trachomatis Test by Ligase Chain Reaction: A Meta-Analysis[J]. Journal of Evidence-Based Medicine, 2012, 12(4): 217-223. DOI: 10.3969/j.issn.1671-5144.2012.04.012
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连接酶链反应检测沙眼衣原体的准确性Meta分析

  • 兰州大学 a. 循证医学中心、基础医学院; b. 第二临床医学院,兰州 730000

基金项目: 2011年度兰州大学中央高校基本科研业务费专项基金资助项目（lzujbky-2011-133）
详细信息
    作者简介:

    葛龙（1988-）, 男, 贵州毕节人, 兰州大学基础医学院 2009 级医学检验专业本科生, 主要研究方向为循证医学。

    通讯作者:

    田金徽,Tel: 0931-8915076, E-mail: tianjh@lzu.edu.cn

  • 中图分类号: R374+.1

The Accuracy for Chlamydia Trachomatis Test by Ligase Chain Reaction: A Meta-Analysis

  • a. Evidence-Based Medicine Center, School of Basic Medical Sciences; b. The Second Clinical Medical College, Lanzhou University, Lanzhou 730000, China

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    摘要
  • 摘要: 目的 评价连接酶链反应技术与培养法诊断沙眼衣原体的准确性。 方法 计算机检索PubMed、EMBASE、Cochrane图书馆、中国生物医学文献数据库、中国期刊全文数据库和万方数据库等,收集连接酶链反应诊断沙眼衣原体的试验研究,依据QUADAS质量评价标准评价纳入研究的质量,采用MetaAnalyst软件进行Meta分析。 结果 共纳入22个研究,共16 073例受试者。Meta分析结果显示: 与诊断沙眼衣原体感染的“金标准”细胞培养相比,连接酶链反应诊断沙眼衣原体感染的合并敏感度、特异度、阳性似然比、阴性似然比、诊断比值比和综合受试者工作特征曲线下面积分别为0.934（95%可信区间0.891~0.961）、0.983（95%可信区间0.972~0.990）、52.88（95%可信区间32.297~86.583）、0.069（95%可信区间0.043~0.110）,1 234.549（95%可信区间545.900 ~2 791.925）和0.986。 结论 连接酶链反应可作为确诊沙眼衣原体感染的一项新技术,在检测沙眼衣原体感染中有着重要意义。
    Abstract: Objective To evaluate the accuracy of ligase chain reaction and culture for Chlamydia trachomatis test. Methods Systematic and comprehensive literatures were searched in PubMed, EMBASE, The Cochrane Library, Chinese Biomedical Literature Database, China National Knowledge Infrastructure and Wanfang database, et al. The studies of ligase chain reaction diagnosis for Chlamydia trachomatis were included. The quality assessment of diagnostic accuracy studies （QUADAS） items were used to assess the quality of the included studies. The MetaAnalyst software was used to analyze the data. Results A total of 22 studies involving 16 073 participants were included. The results of meta-analysis showed that compared with the cell culture, which used considered as gold standard, the ligase chain reaction indicating a higher level of overall accuracy, the summary sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, that the area under the summary receiver operating characteristic （SROC） curve were 0.934（95%CI 0.891~0.961）, 0.983（95%CI 0.972~0.990）, 52.88（95%CI 32.297~86.583）, 0.069（95%CI 0.043~0.110）, 1 234.549（95%CI 545.900~2 791.925） and 0.986, respectively. Conclusion The ligase chain could use as a new technology for Chlamydia trachomatis infection diagnosed, and may be of benefit for testing Chlamydia trachomatis infection.
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    • 参考文献(29)
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  • 收稿日期:  2011-10-07
  • 发布日期:  2012-08-27

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