Abstract: Objective To investigate nursing staff’s cognition and practice of evidence-based nursing, explore improvement measures, and promote the development of evidence-based nursing in our country. Method The questionnaire survey was conducted in a three level of first-class hospital with stratified-cluster sampling method. Result① There were statistical differences among different levels of education （χ²=13.028, P<0.01） or different technical titles （χ²=10.468, P<0.05） of people in understanding of evidence-based nursing. Nurses with higher education and higher title had more awareness of evidence-based nursing. ②Some big barriers in using research evidence in practice were unable to understand statistical principles; lack of enough time for related research; the results were not generalization to expand and research reports were published in English, therefore difficult to understand. ③The effective facilitators in using research evidence in practice were enhancing managerial support research implementation（73.0%）; made study more easier to query（70.8%）; provided colleagues support mechanism（70.1%）. Conclusion Clinical nurses are lack in idea of evidence-based nursing, we should improve the nurses’ own quality, and the management support to promote the evidence-based nursing in the development of our country.