Abstract: Objective To find out the basic conditions of knowledge, attitude and behaviors of evidence-based medicine among Grade 3 students of clinical medicine major at a medical university, discuss the influencing factors and provide the scientific basis for evidence-based medicine teaching reform. Methods All the Grade 3 students of clinical medicine major at a medical university were required to complete questionnaires by themselves. Results About 10% students knew evidence-based medicine, and less than 20% students could answer 4 or more questions of the 7 epidemiology and statistics related questions; about 20% of the students thought journal articles were faithful and true, about 80% of the students considered that clinical experience was the most important, the patients＇ will should be followed, and many measures were lack of strong evidence; about 90% of the students did not take evidence-based medicine related courses, about 90% of the students never searched by PubMed, and when meeting problems in practical about 70% of the students tended to check textbooks and consult teachers. Conclusions About 90% of the students lack of evidence-based medicine knowledge and information retrieval skills, and about 80% of the students lack of evidence-based concept, systemative, effective evidence-based medical education is imperative.