Abstract: Objective To evaluate the correlation between the expression web of Science of 14-3-3ζ protein and gastric cancer. Methods CNKI, VIP, CBM, WanFang Data, PubMed, Cochrane Library and EMBASE,were searched to identify studies on 14-3-3ζ expression for gastric cancer before November 14th,2019. Meta-analysis was performed using RevMan 5.3.5 software after screening the literature, extracting data, and evaluating the risk of bias in the included studies. Results A total of 671 cases of gastric cancer were included in the cross-sectional study. Among them, 14-3-3ζ protein was high expressed in 414 cases and low expressed in 257. Meta-analysis shows that 14-3-3ζ protein was high expressed in gastric cancer tissues （OR=4.94, 95%CI 1.29 to 18.82, P=0.02）, and its high expression was more common in tumors with clinical stage Ⅲ~Ⅳ gastric cancer patients （OR=0.34, 95%CI 0.12 to 0.92, P=0.03）; but with age （OR=0.87, 95% CI 0.59 to 1.28, P=0.48）, gender （OR=1.11, 95%CI 0.72 to 1.69, P=0.64） as well as tumor size （0.74,95%CI 0.30 to 1.84, P=0.02）had no significant relationship. 1-year HR=18.25,95%CI（6.53,51.04）,P<0.000 01,2-year（HR=24.37, 95%CI 9.25 to 64.25, P<0.000 01）,3-year （HR=20.90, 95%CI 7.01 to 62.31, P<0.000 01） and 5-year overall survival rate（HR=19.03, 95%CI 7.02 to 51.62, P<0.000 01） of 14-3-3ζ protein low expression group were significantly higher than high expression group. Conclusion The expression of 14-3-3ζ protein is markedly correlated with clinical stage and the prognosis of gastric cancer.