Abstract: Objective To investigate the effect of programmed cell death 7 (PDCD7) gene on the proliferation and apoptosis of human acute myeloid leukemia Kasumi-1 cells. Methods We transfected Kasumi-1 cells to down regulate the expression of PDCD7 gene with small interfering RNA. Cell proliferation was detected by MTT assay and trypan. Cell morphology was observed by Wright Giemsa staining. Cell cycle was analyzed by flow cytometry with PI staining. Apoptosis was detected by annexin V/PI double staining and Hoechst staining. Apoptosis related proteins NF-κB, bcl-2 and caspase-3 were detected by Western blot. Results The expression of PDCD7 mRNA in Kasumi-1 cells was down regulated by 69% after transfection of PDCD7 siRNA. The proliferation, differentiation, apoptosis and cell cycle of Kasumi-1 cells did not change significantly (P>0.05). Conclusion The proliferation and apoptosis of Kasumi-1 cells were not affected by interference of PDCD7 gene.