Abstract: Objective To study the effects of long non-coding RNA （lncRNA） LINC01515 on the proliferation and apoptosis of non-small cell lung adenocarcinoma cells. Methods The expression levels of LINC01515 in 4 kinds of non-small cell lung adenocarcinoma cells and 1 kind of normal bronchial epithelial cell were detected by qRT-PCR, and the two cell lines with the highest expression levels, H1975 and H838, were selected for subsequent experiments. After transfecting the cells with siLINC01515, we detected the transfection efficiency by qRT-PCR, detected the proliferation activity of each cell lines by WST method, detected apoptosis by flow cytometry, and detected Ki67 and p21 expression levels in the cells by western blot. Results Among the 5 cell lines of H1975, PC-9, H838, H1650 and HBE, H1975 and H838 with the highest expression level of LINC01515 were selected as the research objects. Compared with the control group, the cell proliferation activity of the siLINC01515 group was significantly reduced （P<0.01）, the proportion of cells in the apoptotic phase was significantly increased （P<0.01）, the expression of Ki67 was significantly reduced, and the expression of p21 was significantly increased （both P<0.01）. Conclusions Knockdown of LINC01515 could suppress the proliferation-related protein expression and promote the apoptosis-related protein expression, thereby affecting the proliferation and apoptosis of non-small cell lung adenocarcinoma cells。