Abstract: Objective To investigate the expression of RNA-binding protein heterogeneous nuclear ribonucleoprotein H （hnRNPH1） in glioma and its clinical significance. Methods From January 2000 to December 2012, 311 patients with glioma underwent surgery in Guangdong Provincial People's Hospital were selected. The glioma tissue was taken as the study sample, while the normal brain tissue adjacent to the cancer was taken as the control sample. hnRNPH1 protein extracted from glioma tissues and normal tissues adjacent to cancer was assessed by immunohistochemistry （IHC） in tissue microarray. hnRNPH1 protein expression in pan-canceris analyzed by big bioinformatics database. Chi-square test was used to analyze the relationship between hnRNPH1 expression level in glioma tissues and clinicopathological parameters. Univariate and multivariate regression analysis were used to analyze hnRNPH1 expression level and clinicopathological parameters for predicting survival of patients with glioma. Kaplan-Meier curve was used to analyze the relationship between hnRNPH1 expression level and survival of tumor patients. Results The Cancer Genome Atlas （TCGA） database showed that hnRNPH1 was generally highly expressed in the tumor tissues. Compared with adjacent normal tissues, hnRNPH1 had a higher expression level in glioblastoma tissues, and the expression level of hnRNPH1 gradually increased with the age of patients. Results showed that the expression level of hnRNPH1 was positively correlated with the grade of glioma. Survival analysis showed that patients with high hnRNPH1 expression had a short overall survival. hnRNPH1 expression was not correlated to patient age, sex and tumor location （P>0.05）, while significantly correlated to tumor type and grade （P<0.05）. hnRNPH1 expression level, patient age and tumor grade were independent predictors of prognosis （P>0.05）. Conclusions The expression level of hnRNPH1 in glioma tissue was significantly up-regulated, which was closely related to the poor prognosis of tumor patients and may be involved in the occurrence and development of glioma.