Abstract: Objective To investigate the role of microtubule-associated protein （targeting protein for Xenopus kinesin-like protein 2, TPX2） in the proliferation and metastasis of nasopharyngeal carcinoma and its possible molecular mechanism. Methods The TPX2siRNA plasmid was constructed, and a no-load control was set at the same time. The cells were transfected into human nasopharyngeal carcinoma cells CNE2Z. The cell proliferation was detected by CCK-8 and the cell proliferation was detected by the Transwell test. The expression of cell cycle related proteins and EMT markers were detected by Western blot. Results TPX2siRNA transfection can significantly inhibit the expression of TPX2 in CNE2Z cells. CCK-8 analysis found that the interference of TPX2 significantly inhibited the proliferation of CNE2Z cells. Western blot analysis showed that cyclinD1 and cyclinE were down-regulated and p27 was up-regulated in cyclin. Transwell test analysis found that interference of TPX2 can significantly inhibit cell invasion. Western blot analysis showed that after TPX2 interference, EMT pathway related indicators analysis showed that the epithelial cell molecular marker E-cadherin was up-regulated, and the stromal cell molecular marker Vimentin-N was down-regulated. Conclusions TPX2 has an important effect on the proliferation, migration, and invasion of CNE2Z cells. With the further study of the regulation mechanism of TPX2 in nasopharyngeal carcinoma, TPX2 was expected to become a new target for diagnosis and treatment of nasopharyngeal carcinoma.